Various lysates were subjected to SDS PAGE followed by western blot with 15625-1-AP (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

---

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15625-1-AP (FCGR2A / CD32a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15625-1-AP (FCGR2A / CD32a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human FCGR2A / CD32a (15625-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD16/32 (65057-1-Ig, Clone: 93) and FITC anti-rat IgG2a Antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

---

1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.25 ug FITC-Rat IgG2b isotype control (left) or 0.25 ug FITC Anti-Mouse CD16 / CD32 (FITC-65080, Clone: 2.4G2) (right). Cells were not fixed.

---

1x10^6 mouse splenocytes were stained with 0.5 ug Anti-Mouse CD16 / CD32 (65080-1-Ig, Clone: 2.4G2) and FITC anti-rat IgG2b Antibody at dilution 1:100, or 0.5 ug Control Antibody and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

---

1X10^6 C57BL/6 mouse splenocytes were surface co-stained with FITC Plus Anti-Mouse CD3 (17A2) and 0.2 ug APC Anti-Mouse CD16/32 (APC-65057, Clone: 93) or 0.2 ug APC Rat IgG2a Isotype Control (2A3). Cells were not fixed.

---

1X10^6 mouse splenocytes were surface stained with 0.5 ug Atlantic Blue™ Anti-Mouse CD16/32 (AB-65057, Clone: 93) or 0.5 ug Control Antibody. Cells were not fixed.

---

Various lysates were subjected to SDS PAGE followed by western blot with 25323-1-AP (CD32A/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 405 Anti-Mouse CD16/32 (CL405-65057, Clone:93) or 0.5 ug Isotype Control. Cells were not fixed.

---