Mo7e cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 17425-1-AP (CD33 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD33 antibody (17425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human CD33 (17425-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD33 (65272-1-Ig, Clone:WM-53) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed .

1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD33 (AB-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD33 (CR-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.

Mo7e cells were subjected to SDS PAGE followed by western blot with 67135-2-Ig (CD33 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67135-2-PBS in a different storage buffer formulation.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD33 (CL405-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD33 (CL488-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.