Various lysates were subjected to SDS PAGE followed by western blot with 17425-1-AP (CD33 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of HepG2 cells, using CD33 antibody 17425-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human CD33 (17425-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD33 (65272-1-Ig, Clone:WM-53) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed .

THP-1 cells were subjected to SDS PAGE followed by western blot with 67135-1-Ig (CD33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD33 antibody (67135-1-Ig, Clone: 1B2E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD33 (FITC-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.

CD33 fusion protein Ag11402 SDS-PAGE

THP-1 cells were subjected to SDS PAGE followed by western blot with 13230-1-AP (SIGLEC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 28594-1-AP (Siglec-15 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13454-1-AP (SIGLEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13454-1-AP (SIGLEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).