WB result of FAS/CD95 antibody (13098-1-AP, 1:1000) with si-control and si-FAS transfected Jurkat cells.

Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-FAS/CD95 (IP:13098-1-AP, 3ug; Detection:13098-1-AP 1:500) with HeLa cells lysate 3000ug.

1x10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FAS/CD95 (13098-1-AP) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 Jurkat cells were stained with 0.2ug FAS/CD95 antibody (60196-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human FAS/CD95 (CL488-13098) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

1X10^6 Jurkat cells were stained with 0.2ug FAS/CD95 antibody (60196-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (30601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Standard curve of Human FAS/CD95 ELISA Kit KE00261.

Purity of Recombinant Human Fas was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Immobilized Human Fas (His tag) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand (hFc tag) with a linear range of 1-5 ng/mL.

Various lysates were subjected to SDS PAGE followed by western blot with 10624-2-AP (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (10624-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

WB result of FASN antibody (10624-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells.

IP result of anti-FASN (IP:10624-2-AP, 4ug; Detection:10624-2-AP 1:500) with mouse liver tissue lysate 4000ug.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human FASN (10624-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).