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More validation images available on the product pagePurity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.
Recombinant human TGF beta 1 (HZ-1011) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.01-0.17 ng/mL.
 
Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)
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More validation images available on the product pagePurity of recombinant human BMP-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human BMP-4 (HZ-1045) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-9 ng/ml.
Proteintech BMP-4 (HZ-1045) demonstrates equivalent induction and 2-fold lower EC50 compared to leading competitors. Recombinant human BMP-4 stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-9 ng/ml.
Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)
Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence
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More validation images available on the product pagePurity of recombinant human FGFbasic-TS was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.
Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF basic-TS for 72hrs in defined medium. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.4-2.5 ng/mL.
Proteintech FGF basic-TS (HZ-1285) demonstrates greater induction of proliferation and 3-fold lower EC50 compared to leading competitors. Recombinant human FGFbasic-TS stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.4 ng/mL.
Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence
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More validation images available on the product pagePurity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue
Recombinant human TNF alpha (HZ-1014) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.
 
Proteintech TNF alpha (HZ-1014) demonstrates equivalent induction cytotoxicity and EC50 to leading competitors. TNF alpha demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.
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More validation images available on the product pagePurity of recombinant human IL-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human IL-4 (HZ-1004) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-4 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.07-0.4 ng/mL.
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More validation images available on the product pagePurity of recombinant human IL-6 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue
Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 3G12B10 hybridoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 3G12B10 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 range is 0.03-0.24 ng/mL.
 
Recombinant human IL-6 (HZ-1019) stimulates dose-dependent proliferation of the 7TD1 hybridoma cell line. Cell number was quantitatively assessed by CellTiter® Cell Viability Reagent. 7TD1 cells were treated with increasing concentrations of recombinant IL-6 for 96 hours. The EC50 is <0.5 ng/mL.
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More validation images available on the product pagePurity of recombinant human BMP-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human BMP-2 (HZ-1128) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-2 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 7.5-37.5 ng/ml.
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More validation images available on the product pagePurity of recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
ecombinant human IL-1 beta (HZ-1164) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. 
 
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More validation images available on the product pagePurity of recombinant human VEGF165 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue
Recombinant human VEGF165 (HZ-1038) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF165 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.3-3.75 ng/mL.
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More validation images available on the product pagePurity of recombinant human M-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human M-CSF (Cat no: HZ-1192) stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.
 
Proteintech M-CSF (Cat no: HZ-1192) demonstrates equivalent fold induction and a 2-fold better EC50 compared to leading competitors. Recombinant human M-CSF stimulates dose-dependent proliferation of the murine mouse myloid leukemia (M-NFS-60) cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant M-CSF for 48 hours. The EC50 range is 0.7-4.0 ng/mL.
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More validation images available on the product pagePurity of recombinant human Activin A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.
Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence
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More validation images available on the product pagePurity of recombinant human GM-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
The activity was determined by dose-dependent stimulation of proliferation in human TF-1 cells (human erythroleukemic indicator cell line) measured using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.
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More validation images available on the product pagePurity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
recombinant human IL-2 (HZ-1015) stimulates dose-dependent proliferation of the CTLL-2 (mouse cytotoxic T) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. CTLL-2 cells were starved for 5 hours before treatment with increasing concentrations of GMP recombinant human IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.05-0.35 ng/mL.
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More validation images available on the product pagePurity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue
Recombinant human IFN gamma (HZ-1301) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.
Proteintech IFN gamma (HZ-1301) demonstrates a 3-fold lower EC50 and equivalent dose-dependent inhibition of proliferation to a leading competitor. Recombinant human IFN gamma dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.
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More validation images available on the product pagePurity of recombinant human EGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human EGF (HZ-1326) induces dose-dependent proliferation of the 4MBr-5 (monkey epithelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. 4MBr-5 cells were treated with increasing concentrations of recombinant EGF for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-0.6 ng/mL.
 
 
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Cat No. HZ-1204
GMP-grade available on request
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More validation images available on the product pagePurity of recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human VEGF121 (HZ-1204) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF121 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is less than 15 ng/mL.
 
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More validation images available on the product pagePurity of recombinant human SCF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue
Recombinant human SCF (HZ-1024) stimulates dose-dependent proliferation of the MO7e human megakaryoblastic leukemia cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of GMP recombinant SCF for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 15-85 ng/mL.
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More validation images available on the product pagePurity of recombinant human TGF beta 3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human TGF beta 3 (HZ-1090) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.14-0.75 ng/mL
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More validation images available on the product pagePurity of recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human FLT3 (HZ-1151) Ligand stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range is 0.4-3 ng/mL. 
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More validation images available on the product pagePurity of recombinant human HGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
The activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5 using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.
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More validation images available on the product pagePurity of recombinant human IL-3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human IL-3 (HZ-1074) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.4-2.0 ng/mL