Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 IL-2 antibody (CL488-60306, Clone: 2F11C5 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 IL-2 antibody (CL488-60306, Clone: 2F11C5 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 IL-2 antibody (CL594-60306, Clone: 2F11C5 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 IL-2 antibody (CL594-60306, Clone: 2F11C5 ) at dilution of 1:200.

Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP-grade recombinant human IL-2 (HZ-1015-GMP) stimulates dose-dependent proliferation of the CTLL-2 (mouse cytotoxic T) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. CTLL-2 cells were starved for 5 hours before treatment with increasing concentrations of GMP recombinant human IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.05-0.35 ng/mL.

Standard curve of Mouse IL-2 ELISA Kit KE10100.

Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

recombinant human IL-2 (HZ-1015) stimulates dose-dependent proliferation of the CTLL-2 (mouse cytotoxic T) cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. CTLL-2 cells were starved for 5 hours before treatment with increasing concentrations of GMP recombinant human IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.05-0.35 ng/mL.

Various lysates were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

PMA, A23187 and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 26156-1-AP (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26156-1-AP (Il2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26156-1-AP (Il2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).