• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-2 protein- GMP grade

EC50

0.05-0.35 ng/mL EC50

Specific Activity

1.37 x 10^7 IU/mg calibrated against IL-2 reference standard (NIBSC cat no: 86/500). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1015-GMP

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF



Technical Specifications

GeneID 3558
Species Human
Expression HEK293
EC50 0.05-0.35 ng/mL EC50
Specific Activity1.37 x 10^7 IU/mg calibrated against IL-2 reference standard (NIBSC cat no: 86/500). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP60568
Molecular Mass 15 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF