|Positive WB detected in||Jurkat cells, Human white blood cells (WBC)|
|Positive IHC detected in||human tonsillitis tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
60306-1-Ig targets IL-2 in WB, IHC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||IL-2 fusion protein Ag10199|
|Full Name||interleukin 2|
|Calculated molecular weight||153 aa, 18 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC070338|
|Gene ID (NCBI)||3558|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Alex (Verified Customer) (10-11-2019)
IL-2 Staining is dirty in mouse colon, attempted various dilutions and retrieval methods none of which worked.