Tested Applications
| Positive WB detected in | Jurkat cells, MOLT-4 cells, human peripheral blood leukocyte |
| Positive IHC detected in | human tonsillitis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60306-1-Ig targets IL-2 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig, hamster |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10199 Product name: Recombinant human IL-2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-153 aa of BC070338 Sequence: MYRMQLLSCIALSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT Predict reactive species |
| Full Name | interleukin 2 |
| Calculated Molecular Weight | 153 aa, 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC070338 |
| Gene Symbol | IL2 |
| Gene ID (NCBI) | 3558 |
| RRID | AB_2881420 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P60568 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.
Publications
What published studies show
The IL-2 Monoclonal antibody (2F11C5), catalog number 60306-1-Ig, has 15 citations in journals including J Am Chem Soc, Nucleic Acids Res, Acta Pharm Sin B, J Neuroinflammation, Free Radic Biol Med, Transl Psychiatry, Front Immunol, Cancer Med, PLoS One, and others. Research fields span immunology, oncology, neuroinflammation, type 1 diabetes, gut microbiota, oxidative stress, dermatology, and hematology.
| Species | Application | Title |
|---|---|---|
J Am Chem Soc A Granzyme B-Cleavable T Cell-Targeted Bispecific Cell Vesicle Connector for Reversing New-Onset Type 1 Diabetes | ||
Nucleic Acids Res FOXC2 represses NFAT1-dependent transcription through a DNA-facilitated protein-protein interaction. | ||
Acta Pharm Sin B Development of a bacteria-nanosapper for the active delivery of ZIF-8 particles containing therapeutic genes for cancer immune therapy | ||
J Neuroinflammation LAG3 limits regulatory T cell proliferation in α-synuclein gut-to-brain transmission model | ||
Acta Biomater Bioorthogonal and synthetic biology-engineered bacterial outer membrane vesicles for enhanced photo-immunotherapy. | ||
Free Radic Biol Med Oxidative stress-induced IL-15 transtrans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. |
Reviews
What customers say
The single review for this product (rated 1/5) reports a negative experience with IHC on mouse colon tissue at a 1:2000 dilution. The reviewer found the IL-2 staining to be dirty and noted that trying various dilutions and antigen retrieval methods did not resolve the issue. Overall, the feedback suggests the antibody performed poorly in this specific application and tissue type.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alex (Verified Customer) (10-11-2019) | IL-2 Staining is dirty in mouse colon, attempted various dilutions and retrieval methods none of which worked.
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