• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-2 protein

Activity

0.05-0.35 ng/mL EC50

Specific Activity

1.37x10^7 IU/mg calibrated against IL-2 reference standard (NIBSC cat no: 86/500). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1015

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF



Technical Specifications

GeneID 3558
Species Human
Expression HEK293
Activity 0.05-0.35 ng/mL EC50
Specific Activity1.37x10^7 IU/mg calibrated against IL-2 reference standard (NIBSC cat no: 86/500). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP60568
Molecular Mass 15 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

Publications

SpeciesTitle
human

Nat Med

A human anti-IL-2 antibody that potentiates regulatory T cells by a structure-based mechanism.

Authors - Eleonora Trotta
mouse

Nat Med

Recurrent ECSIT mutation encoding V140A triggers hyperinflammation and promotes hemophagocytic syndrome in extranodal NK/T cell lymphoma.

Authors - Haijun Wen
human

J Mol Cell Cardiol

Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis.

Authors - MakikoOhshima
human

Stem Cell Res Ther

hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathway.

Authors - Aiping Zhang
human

J Cell Mol Med

Evaluation of piggyBac-mediated anti-CD19 CAR-T cells after ex vivo expansion with aAPCs or magnetic beads.

Authors - Li-Rong Yang
human

Clin Immunol

Therapeutic polyclonal human CD8+ CD25+ Fox3+ TNFR2+ PD-L1+ regulatory cells induced ex-vivo.

Authors - David AHorwitz