Various lysates were subjected to SDS PAGE followed by western blot with 14794-1-AP (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14794-1-AP (NDUFB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (14794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.
IP result of anti-NDUFB8 (IP:14794-1-AP, 4ug; Detection:14794-1-AP 1:2000) with HepG2 cells lysate 880 ug.
WB result of NDUFB8 antibody (14794-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells.