Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WB result of 12998-1-AP.

IP Result of anti-NF-L (IP:12998-1-AP, 4ug; Detection:12998-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human NF-L (12998-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NF-L antibody (12998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NF-L antibody (12998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NF-L antibody (12998-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-L (60189-1-Ig, Clone:5C12G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 60189-1-Ig (NF-L antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60189-1-Ig (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®488 NF-L antibody (CL488-60189, Clone: 5C12G4 ) at dilution of 1:200.

Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 NF-L antibody (CL488-12998) at dilution of 1:200.

1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NF-L (CL488-12998) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 NF-L antibody (CL594-60189, Clone: 5C12G4 ) at dilution of 1:200.

1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NF-L (CL594-60189, Clone:5C12G4) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0044 (NF-L IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0044 (NF-L IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60189-1-Ig (NF-L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-L (60189-1-Ig, Clone:5C12G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 60189-1-Ig (NF-L antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60189-1-Ig (NF-L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60189-1-PBS in a different storage buffer formulation.