Various lysates were subjected to SDS PAGE followed by western blot with 17519-1-AP (RIPK1-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17519-1-AP (RIPK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Various lysates were subjected to SDS PAGE followed by western blot with 66854-1-Ig (Phospho-RIPK1 (Ser161) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was untreated (left) or treated with Lambda Protein Phosphatase (λ-PPase, 500U, right) at 37℃ for 1 hours before blocking.

Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66854-1-Ig (RIPK1 phospho S161 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Phospho-RIPK1 (Ser161) antibody (66854-1-Ig, Clone: 1B2G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.13 ug Anti-Human Phospho-RIPK1 (Ser161) (66854-1-Ig, Clone:1B2G1) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Non-treated HT-29 and TNF alpha (HZ-1014) treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28252-1-AP (Phospho-RIPK1 (Ser166) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Various lysates were subjected to SDS PAGE followed by western blot with 29932-1-AP (RIPK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite®488 Phospho-RIPK1 (Ser161) antibody (CL488-66854, Clone: 1B2G1 ) at dilution of 1:100.

1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite488 Anti-Human Phospho-RIPK1 (Ser161) (CL488-66854, Clone:1B2G1), or 0.13 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®488 Anti-Human Phospho-RIPK1 (Ser161) (CL488-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Human Phospho-RIPK1 (Ser161) (CL647-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 647 Phospho-RIPK1 (Ser161) antibody (CL647-66854, Clone: 1B2G1 ) at dilution of 1:400.

1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®555 Anti-Human Phospho-RIPK1 (Ser161) (CL555-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®594 Anti-Human Phospho-RIPK1 (Ser161) (CL594-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.