Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40x lens).
WB result of SDHB antibody (10620-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SDHB transfected HepG2 cells.
IP result of anti-SDHB (IP:10620-1-AP, 4ug; Detection:10620-1-AP 1:5000) with HEK-293 cells lysate 1360 ug.
Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SDHB antibody (10620-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SDHB (10620-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).