Human milk sample were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27064-1-AP (TRAIL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).