Immunofluorescent staining of TUBB3 (66375-1-lg, 1:250) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (Green: TUBB3; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and PAX6 (12323-1-AP) at 1:500. Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue).

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66375-1-Ig (TUBB3-specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 26975-1-AP (NeuN antibody, red).

Various lysates were subjected to SDS PAGE followed by western blot with 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:49000 incubated at room temperature for 1.5 hours.

1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human TUBB3-specific (66375-1-Ig, Clone:1F8G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 40x lens).

Western blot analysis of TUBB3 in various tissues and cell lines with 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with CL488-66240 (Beta Tubulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66240 (beta Tubulin antibody) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Beta Tubulin (CL488-66240, Clone:1D4A4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

HeLa cells were subjected to SDS PAGE followed by western blot with HRP-66240 (beta Tubulin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Neuro-2a lysates were subjected to SDS PAGE followed by western blot with CL594-66375 (TUBB3-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66375 (TUBB3-specific antibody) at dilution of 1:50.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66375 (TUBB3-specific antibody) at dilution of 1:50.

1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TUBB3-specific (CL594-66375, Clone:1F8G10) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TUBB3-specific antibody (CL594-66375, Clone: 1F8G10 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using the CoraLite®594-conjugated version of this antibody, CL594-66240 (beta Tubulin antibody), at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Beta Tubulin (CL594-66240, Clone:1D4A4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Beta Tubulin (CL488-10094) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Beta Tubulin antibody (CL488-80713, Clone: 2O13 ) at dilution of 1:200.

E14.5 FFPE mouse spine stained for beta-III tubulin (red, Cat. No CL488-66375) and GFAP (cyan, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to red. GFAP stains astrocytes along the spinal column. In this image, astrocyte projections can be seen among spinal neurons in the spinal cord. Image credit: @Immunofluorescence on Instagram.

Neonatal mouse cerebellum stained for beta-III tubulin (magenta, Cat. No CL488-66375) and GFAP (green, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to magenta. GFAP stains astrocytes. In this image, astrocyte projections can be seen intermingling with neurons. Image credit: @Immunofluorescence on Instagram.

Adult mouse testes were co-stained with TUBB3 (CL488-66375, in green), DDX4/VASA (51042-1-AP, in magenta), and PCNA (60097-1-Ig, in blue). TUBB3/Beta III tubulin marks the Sertoli cells (structural support cells), VASA marks the germ cells (developing sperm), and PCNA marks proliferating cells (these cells are at the base of the tubule, where the sperm stem cells are located). The image was created in paid partnership with @Immunofluorescence on Instagram.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66375 (TUBB3-specific antibody) at dilution of 1:50.

Neuro-2a cell lysates were subjected to SDS PAGE followed by western blot with CL488-66375 (TUBB3-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.