Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, red) and anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green) and anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, blue), anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, yellow) and anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 750 Kit (KFA024, grey).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Ki67 labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green) and anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green) and DAPI (blue).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021).

WB of HEK-293 cell lysates: HEK-293 cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, red) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green).

FlexAble Workflow

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-PAF49 labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, yellow), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta), CoraLite® 594-conjugated GNL3 antibody (CL594-67169) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), mouse anti-Lamin and anti-mouse IgG secondary antibody Alexa Fluor® 568 (red), rabbit anti-COXIV (11242-1-AP) and anti-rabbit IgG secondary antibody Alexa Fluor® 647 (cyan) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green). Cell nuclei are in blue. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 7505 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041).

WB of HeLa and HepG2 cell lysates: HeLa and HepG2 cell lysates were detected with anti-HSP90 (60318-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-CACYBP (68161-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-TUFM (67802-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green). Nuclei were stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), mouse IgG2b anti-PAF49 primary and anti-mouse IgG secondary antibody Alexa Fluor® 568 (red) and CoraLite® 647-conjugated HDAC2 antibody (CL647-67615, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), mouse IgG2b anti-Lamin primary and anti-mouse IgG secondary antibody Alexa Fluor® 568 (red) and anti-Tom20 (66777-1-Ig) labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, magenta). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, yellow). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA061).​

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA061).​

WB of HeLa and HEK-293 cell lysates: HeLa and HEK-293 Cell lysates were detected with anti-Beta Actin (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 Kit (KFA061, blue); anti-Alpha Tubulin (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA062, red);  and anti-ANP32B (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 Kit (KFA064, green).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tom70 (14528-1-AP) labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, green) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP43 (10782-2-AP) labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, green), anti-TOM20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red), anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and anti-CD147 labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, cyan).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP-43 (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM70 (14528-1-AP) labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, green) and DAPI (blue).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, cyan) or with isotype control antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, red).

WB of HEK-293 cell lysates: HEK-293 cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024, red).

FlexAble Workflow

Live A431 cells were immunostained with anti-EGFR (cetuximab biosimilar) labeled with FlexAble CoraLite Plus 488 Antibody Labeling Kit for Human IgG (KFA104). Nuclei are in blue.  Epifluorescence images were acquired with a 20x objective and post-processed.​

Live SKBR-3 cells immunostained with anti-HER2 (trastuzumab biosimilar) labeled with FlexAble CoraLite Plus 488 Antibody Labeling Kit for Human IgG (KFA104).  Nuclei are in blue.  Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble CoraLite® Plus 488 Kit (KFA104) and anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with PE  (PE-65197).

Immunofluorescence of A431 cells: PFA-fixed cells were stained with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue).  Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green) and rat IgG2b kappa anti-RNA PolymeraseII antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, red).  Epifluorescence images were acquired with a 20x objective and post-processed.

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). ​ Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 488 for Rat Kappa Light Chain (KFA121) and CD3 antibody (CL750-65151).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble CoraLite® Plus 488 for Rat Kappa Light Chain (KFA121). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 488 Kit (KFA121) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta) and with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue).   Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.