Immunofluorescence of human kidney: FFPE human kidney sections were stained with anti-Calbindin (14479-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, yellow), anti-ACE2 (66699-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta), CoraLite®488-conjugated Podocalyxin antibody (CL488-18150, green) and DAPI (blue).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, yellow), anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan) and DAPI (blue). ​Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Vimentin labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, yellow) and anti-hnRNP labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024).

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023) and anti-CD8 (clone RPA-T8, 65144-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).

WB of A431 cell lysate: siRNA transfected A431 cell lysates were detected with anti-P53 (80077-1-RR) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, green) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta), CoraLite® 594-conjugated GNL3 antibody (CL594-67169) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-lg) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-Actin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM70 (14528-1-AP) labeled with FlexAble CoraLite® Plus 488 Kit (KFA001, green), anti-GNL3 (67169-1-lg) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-Actin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta). Cell nuclei are in cyan. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-PAF49 labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, yellow), anti-Lamin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta) and DAPI (blue).  Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Actin labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, magenta) and DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 6475 Kit (KFA043).

WB of HeLa and HepG2 cell lysates: HeLa and HepG2 cell lysates were detected with anti-HSP90 (60318-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-CACYBP (68161-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-TUFM (67802-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue).

WB of HeLa and HepG2 cell lysates: HeLa and HepG2 cell lysates were detected with anti-TUFM (67802-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA042, red) and anti-CACYBP (68161-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA043, blue). 

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tom20 (66777-1-Ig) labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, magenta). Nuclei were stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HDAC2 (676165-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), mouse IgG2b anti-Lamin primary and anti-mouse IgG secondary antibody Alexa Fluor® 568 (red) and anti-Tom20 (66777-1-Ig) labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, magenta). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA061, green), anti-Lamin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, yellow). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with CoraLite® 488-conjugated Tom20 antibody (CL488-66777, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti- PAF49 antibody labeled with FlexAble ​CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Actin antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA041, green), anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-Lamin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA063, grey). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA063).​

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD20 (clone 2G7, 65085-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA063).​

Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-MAP2 (17490-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, orange), anti-GFAP (16825-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and CoraLite®488-conjugated TDP-43 antibody (CL488-10782, green).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-COXIV (11242-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, yellow), anti-GM130 (11308-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and DAPI (blue).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP-43 (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-Tom20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red) and anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were stained with 0.5 µg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, cyan) or with isotype control antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red).

WB of HeLa cell lysates: HeLa cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 (29824-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

FlexAble Workflow

Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite® 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in frist step, anti-CD45 antibody (80297-1-RR) labeled  with CoraLite® Plus 555 (KFA002, orange) in sencond step, anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite® 647 Kit (KFA003, magenta) in the third step, and DAPI (blue).

Live A431 cells were immunostained with anti-EGFR (cetuximab biosimilar) labeled with FlexAble CoraLite Plus 647 Antibody Labeling Kit for Human IgG (KFA106). Nuclei are in blue.​  Epifluorescence images were acquired with a 20x objective and post-processed.​

Live SKBR-3 cells immunostained with anti-HER2 (trastuzumab biosimilar) labeled with FlexAble CoraLite Plus 647 Antibody Labeling Kit for Human IgG (KFA106).  Nuclei are in blue.  Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble CoraLite® Plus 647 Kit (KFA106) and anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with PE (PE-65197).

Immunofluorescence of A431 cells: PFA-fixed cells were stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta). Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2b kappa anti-RNA Polymerase II antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431: Formaldehyde-fixed A431 cells were stained with rat IgG2a kappa anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 555 Kit (KFA122, yellow) and rat IgG1 kappa anti-RPA32 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta).  Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with rat anti-RNA Pol II Ser5 antibody labeled with FlexAble CoraLite® Plus 647 Kit (KFA123, magenta) and with rat anti-Tubulin alpha antibody labeled with FlexAble CoraLite® Plus 488 Kit (KFA121, green). Cell nuclei were stained with DAPI (blue).   Confocal images were acquired with a 63x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescent analysis of FFPE mouse colon tissue slides with Rat anti-mouse Cdh1 antibody labeled with FlexAble CoraLite Plus 647 Kit (KFA123, magenta). With/without quencher. Cell nuclei were stained with DAPI (blue).

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). The cells were surface stained with CD3 antibody (CL488-65151) before fixation. Then, cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 647 kit (KFA123).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1) or rat IgG2a isotype control (65209-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed. Histogram, red: Rat IgG2a isotype control (65209-1-Ig); blue: Rat anti-mouse CD86 (65068-1-Ig).

1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite Plus 488 Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 647 (KFA123). Cells were not fixed.

Human peripheral blood lymphocytes (PBMC) were either un-stimulated (left) or stimulated with PMA and Ionomycin for 6 hours in the presence of monensin (right). ​ Cells were fixed and permeabilized with Foxp3/Transcription Factor Staining Buffer Kit (PF00011). After wash, cells were stained with rat anti-human TNF-α antibody labeled with FlexAble CoraLite® Plus 647 for Rat Kappa Light Chain (KFA123) and CD3 antibody (CL488-65151).

1X10^6 of LPS treated mouse splenocytes were surface stained with rat anti-Mouse CD86 (65068-1-Ig, Clone: GL1, red) or rat IgG2a isotype control (65209-1-Ig, blue) labeled with FlexAble CoraLite® Plus 647 for Rat Kappa Light Chain (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 488 Kit (KFA121) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble FITC Plus Kit (KFA126) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 555 Kit (KFA122) and 0.5µg rat anti-Mouse CD3 (65077-1-Ig, Clone: 17A2) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with rat anti-Mouse CD3 (CL488-65077, Clone: 17A2) and 0.5µg rat anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) labeled with FlexAble CoraLite® Plus 647 Kit (KFA123). Cells were not fixed.