Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 750 Kit (KFA024, cyan) and DAPI (blue).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024).

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD45 antibody (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024).

WB of HEK-293 cell lysates: HEK-293 cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024, red).

FlexAble Workflow

PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044, grey). Epifluorescence images were acquired with a 20x objective and post-processed.​

PFA-fixed HeLa cells were stained with anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044, grey) and Sytox Deep Red (cell nuclei, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044).​

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA066, cyan) and anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA064, red). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tom20 (66777-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA062, red) and anti-PAF49 antibody labeled with FlexAble CoraLite® Plus 750 Kit (KFA064, grey). Cell nuclei were stained with DAPI (blue).  Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble FITC Plus Kit (KFA028) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA064).  Cells are not fixed, lymphocytes are gated.

Western Blot of HeLa and HEK-293 cell lysates were detected with anti-Beta Actin antibody (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 (KFA061) and anti-ANP32B antibody (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064).

Western Blot of HeLa and HEK-293 cell lysates were detected with anti-ANP32B antibody (66160-1-Ig) labeled with FlexAble CoraLite Plus 488 (KFA061) and anti-Beta Actin antibody (66009-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064).

Western Blot of HeLa and Jurkat cell lysates were detected with anti-Alpha Tubulin antibody (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 (KFA062) and anti-FDPS antibody (67972-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064).

Western Blot of HeLa and Jurkat cell lysates were detected with anti-Alpha Tubulin antibody (66031-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064) and anti-FDPS antibody (67972-1-Ig) labeled with FlexAble CoraLite Plus 555 (KFA062).

Western Blot of HeLa and HEK-293 cell lysates were detected with anti-Beta Actin antibody (66009-1-Ig) labeled with FlexAble CoraLite Plus 488 (KFA061), anti-Alpha Tubulin antibody (66031-1-Ig) labeled with FlexAble CoraLite Plus 555 (KFA062), and anti-ANP32B antibody (66160-1-Ig) labeled with FlexAble CoraLite Plus 750 (KFA064).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red) and anti-TOM20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, cyan).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP43 (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit (KFA001, green), anti-TOM20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit (KFA002, red), anti-Lamin B1 (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, magenta) and anti-CD147 labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, cyan).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM20 (11802-1-AP) labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, cyan) and DAPI (blue).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of Jurkat cells. 1X10^6 Jurkat cells were stained with 0.5 µg anti-HSP90 antibody (13171-1-AP) labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, cyan) or with isotype control antibody labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, red).

WB of HEK-293 cell lysates: HEK-293 cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, red) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® 488 Kit (KFA021, green).

FlexAble Workflow

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble CoraLite® Plus 750 Kit (KFA107) and anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with PE (PE-65197).

Rat, mouse, pig, and human blood lysate were subjected to SDS PAGE followed by western blot with FlexAble CoraLite Plus 750 for Human IgG at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Live A431 cells were immunostained with anti-EGFR (cetuximab biosimilar) labeled with FlexAble CoraLite Plus 750 Antibody Labeling Kit for Human IgG (KFA107). Nuclei are in blue.​  Epifluorescence images were acquired with a 20x objective and post-processed.​

Live SKBR-3 cells immunostained with anti-HER2 (trastuzumab biosimilar) labeled with FlexAble CoraLite Plus 750 Antibody Labeling Kit for Human IgG (KFA107).  Nuclei are in blue.  Epifluorescence images were acquired with a 20x objective and post-processed.