Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of HepG2 cells, using SHH antibody 20697-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB.

IP Result of anti-SHH (IP:20697-1-AP, 4ug; Detection:20697-1-AP 1:500) with mouse liver tissue lysate 6400ug.

Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SHH (20697-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

human kidney tissue were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13388-1-AP (IHH Antibody) at dilution of 1:200 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 13388-1-AP (IHH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13889-1-AP (DHH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Purity of recombinant human SHH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human SHH (HZ-1306) stimulates dose-dependent induction of alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. C3H10T1/2 cells were treated with increasing concentrations of recombinant human SHH for 120 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values are no more than 350 ng/mL.
 

Various lysates were subjected to SDS PAGE followed by western blot with 29466-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HHIP antibody (29466-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 29466-1-AP (HHIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0135 (IHH IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC0135 (IHH IHC Kit).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0134 (SHH IHC Kit).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC0134 (SHH IHC Kit).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC0134 (SHH IHC Kit).

Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.