IHH Antibody

IHH Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13388-1-AP

Synonyms

BDA1, HHG 2, HHG2, IHH, Indian hedgehog protein



Tested Applications

Positive WB detected inhuman kidney tissue, human heart tissue
Positive IHC detected inhuman kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

13388-1-AP targets IHH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IHH fusion protein Ag4046
Full Name Indian hedgehog homolog (Drosophila)
Calculated molecular weight 411aa,45 kDa
Observed molecular weight 45 kDa
GenBank accession number BC034757
Gene symbol IHH
Gene ID (NCBI) 3549
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IHH antibody 13388-1-APDownload protocol
IHC protocol for IHH antibody 13388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Dev Biol

DDB1 promotes the proliferation and hypertrophy of chondrocytes during mouse skeleton development.

Authors - Lianzheng Zhao
mouseWB,IHC

Int J Mol Sci

Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism.

Authors - Hongxu Yang
mouseIHC

Osteoarthritis Cartilage

mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis.

Authors - H Zhang
humanIHC

Mol Ther

Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2.

Authors - Hua Wang
mouseIF

Cell Mol Life Sci

Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway.

Authors - Takeshi Oichi
humanWB

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu