IHH Polyclonal antibody

IHH Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13388-1-AP

Synonyms

BDA1, HHG 2, HHG2, IHH, Indian hedgehog protein



Tested Applications

Positive WB detected inhuman kidney tissue, HEK-293 cells, human heart tissue
Positive IHC detected inhuman kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13388-1-AP targets IHH in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IHH fusion protein Ag4046
Full Name Indian hedgehog homolog (Drosophila)
Calculated Molecular Weight 411 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC034757
Gene Symbol IHH
Gene ID (NCBI) 3549
RRIDAB_2248725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IHH antibody 13388-1-APDownload protocol
IHC protocol for IHH antibody 13388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu
mouseIF

Cell Mol Life Sci

Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway.

Authors - Takeshi Oichi
humanIHC

Mol Ther

Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2.

Authors - Hua Wang
mouseIHC

Osteoarthritis Cartilage

mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis.

Authors - H Zhang
mouseWB,IHC

Int J Mol Sci

Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism.

Authors - Hongxu Yang
mouseIHC

Dev Biol

DDB1 promotes the proliferation and hypertrophy of chondrocytes during mouse skeleton development.

Authors - Lianzheng Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hongxue (Verified Customer) (05-13-2022)

WB works well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse liver
IHH Antibody Western Blot validation (1:1000 dilution) in Mouse liver (Cat no:13388-1-AP)