DHH Polyclonal antibody
DHH Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
Cat no : 13889-1-AP
|Positive WB detected in||mouse skeletal muscle tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
13889-1-AP targets DHH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DHH fusion protein Ag4850|
|Full Name||desert hedgehog homolog (Drosophila)|
|Calculated molecular weight||396 aa, 44 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC033507|
|Gene ID (NCBI)||50846|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Desert hedgehog (DHH) is one of three ligands in the hedgehog signaling pathway that controls gonad
morphogenesis and nerve perineurium differentiation.
What is the molecular weight of DHH? Is DHH post-translationally modified?
DHH protein is synthesized in a precursor form of ~45 kDa. Hedgehog proteins contain a small N-terminal signal
peptide, which is cleaved off intracellularly, leading to 39 kDa species. Hedgehog proteins are then subject to
autoproteolysis, which leads to the generation of a 19 kDa N-terminal fragment with added cholesterol moiety
and a 25 kDa C-terminal fragment (PMID: 7985023). The C-terminal fragment catalyzes the precursor proteolysis,
while the N-terminal fragment gets secreted from cells and acts as an active ligand in the hedgehog signaling
What is the subcellular localization of DHH?
DHH protein is subject to autoproteolysis, as described above, and the N-terminal fragment is secreted from
cells in a regulated manner. The amounts of extracellular hedgehog proteins determine local and long-range
signaling (PMID: 7736596 and 7885476).
What is the tissue expression pattern of DHH?
Unlike other hedgehog ligands, expression of DHH is very tissue-specific. DHH is strongly expressed in Steroli
cell precursors, adult sterol cells, and in Leydig cells in testis (PMID: 8805249, 12050120) but also in granulosa
cells in ovaries (PMID: 15878962), as well as in Schwann cells-glia of the peripheral nervous system
What is the role of DHH in morphogenesis?
DHH plays a role in the correct development of male gonads and perineurium. Loss of DHH leads to peripheral
neuropathy and gonadal dysgenesis (PMID: 11017805).
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