• Featured Product
  • KD/KO Validated

SHH Polyclonal antibody

SHH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20697-1-AP

Synonyms

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS



Tested Applications

Positive WB detected inHeLa cells, rat kidney tissue, HepG2 cells, LNCaP cells, PC-3 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue, human pancreas cancer tissue, human ovary tumor tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse kidney tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20697-1-AP targets SHH in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, mink
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sonic hedgehog homolog (Drosophila)
Calculated molecular weight 50 kDa
Observed molecular weight 45-51 kDa
GenBank accession numberNM_000193
Gene symbol SHH
Gene ID (NCBI) 6469
RRIDAB_10694828
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sonic hedgehog (SHH) is a member of the Hedgehog (Hh) family of proteins that control cell growth, survival, 

and fate.


What is the molecular weight of SHH?

The molecular weight of SHH is approximately 45 kDa, but it is cleaved autocatalytically to generate a 20 kDa 

N-terminal fragment and a 25 kDa C-terminal fragment responsible for hedgehog biological activity and the 

auto-processing machinery respectively (PMID: 10753901).


What is the tissue specificity of SHH?

SHH is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate development, 

SHH is expressed in midline tissues such as the node, notochord, and floor plate to control the left-right and 

dorso-ventral patterning of the embryo (PMID: 12729563). SHH is expressed in fetal intestine, liver, kidney, 

and lung tissue, but is not expressed in adult tissues.


What is the cellular localization of SHH?

SHH is localized to the cell membrane. The N-terminal fragment is associated with lipid rafts at the cell surface, 

whereas the C-terminal fragment diffuses from the cell.


What is the role of SHH in the Sonic hedgehog signaling pathway?

This pathway is one of the major trafficking networks that regulate key events during developmental growth 

and patterning (PMID: 19368798). Loss of SHH signaling and regulation has been implicated in birth defects, 

tissue regeneration, stem cell renewal, and cancer growth (PMID: 15549094). The regulatory action of the SHH 

pathway is linked to the secretion, uptake, and translocation of the SHH protein; a ligand precursor, which is 

implicated in the regulation of the central nervous system polarity and neural patterning (PMID: 11130178, 

11801325).


Protocols

Product Specific Protocols
WB protocol for SHH antibody 20697-1-APDownload protocol
IHC protocol for SHH antibody 20697-1-APDownload protocol
IF protocol for SHH antibody 20697-1-APDownload protocol
IP protocol for SHH antibody 20697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu
mouseIHC

Theranostics

Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development.

Authors - Wei Ge
humanWB

Cancer Lett

An SGLT2 inhibitor modulates SHH expression by activating AMPK to inhibit the migration and induce the apoptosis of cervical carcinoma cells.

Authors - Zipeng Xie
  • KD Validated
humanWB,IF

Oxid Med Cell Longev

TLR4 Modulates Senescence and Paracrine Action in Placental Mesenchymal Stem Cells via Inhibiting Hedgehog Signaling Pathway in Preeclampsia.

Authors - Yanqi Zhong
mouseWB

Phytother Res

Total flavonoid extract from Dracocephalum moldavica L. improves pulmonary fibrosis by reducing inflammation and inhibiting the hedgehog signaling pathway

Authors - Cheng-Hui He
humanWB

Int J Oncol

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Authors - Wei Jia