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- KD/KO Validated
SHH Polyclonal antibody
SHH Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 20697-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, rat kidney tissue, HepG2 cells, LNCaP cells, PC-3 cells, mouse liver tissue, rat liver tissue
|Positive IP detected in
|mouse liver tissue
|Positive IHC detected in
|human liver cancer tissue, mouse kidney tissue, human pancreas cancer tissue, human ovary tumor tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HepG2 cells, mouse kidney tissue
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:3000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:10-1:100
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
20697-1-AP targets SHH in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, mink
|Host / Isotype
|Rabbit / IgG
|sonic hedgehog homolog (Drosophila)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Sonic hedgehog (SHH) is a member of the Hedgehog (Hh) family of proteins that control cell growth, survival,
What is the molecular weight of SHH?
The molecular weight of SHH is approximately 45 kDa, but it is cleaved autocatalytically to generate a 20 kDa
N-terminal fragment and a 25 kDa C-terminal fragment responsible for hedgehog biological activity and the
auto-processing machinery respectively (PMID: 10753901).
What is the tissue specificity of SHH?
SHH is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate development,
SHH is expressed in midline tissues such as the node, notochord, and floor plate to control the left-right and
dorso-ventral patterning of the embryo (PMID: 12729563). SHH is expressed in fetal intestine, liver, kidney,
and lung tissue, but is not expressed in adult tissues.
What is the cellular localization of SHH?
SHH is localized to the cell membrane. The N-terminal fragment is associated with lipid rafts at the cell surface,
whereas the C-terminal fragment diffuses from the cell.
What is the role of SHH in the Sonic hedgehog signaling pathway?
This pathway is one of the major trafficking networks that regulate key events during developmental growth
and patterning (PMID: 19368798). Loss of SHH signaling and regulation has been implicated in birth defects,
tissue regeneration, stem cell renewal, and cancer growth (PMID: 15549094). The regulatory action of the SHH
pathway is linked to the secretion, uptake, and translocation of the SHH protein; a ligand precursor, which is
implicated in the regulation of the central nervous system polarity and neural patterning (PMID: 11130178,
|Product Specific Protocols
|WB protocol for SHH antibody 20697-1-AP
|IHC protocol for SHH antibody 20697-1-AP
|IF protocol for SHH antibody 20697-1-AP
|IP protocol for SHH antibody 20697-1-AP
|Click here to view our Standard Protocols
TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.
Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development.
An SGLT2 inhibitor modulates SHH expression by activating AMPK to inhibit the migration and induce the apoptosis of cervical carcinoma cells.
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TLR4 Modulates Senescence and Paracrine Action in Placental Mesenchymal Stem Cells via Inhibiting Hedgehog Signaling Pathway in Preeclampsia.
Total flavonoid extract from Dracocephalum moldavica L. improves pulmonary fibrosis by reducing inflammation and inhibiting the hedgehog signaling pathway
Int J Oncol
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.