1x10^6 human PBMCs were surface stained with 0.125 ug Anti-Human CD2 (TS1/8) Mouse Recombinant Antibody (65565-1-MR, Clone: TS1/8) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were then stained with FITC Plus Anti-Human CD19 (HIB19) (FITC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD64 (10.1) Mouse Recombinant Antibody (65572-1-MR, Clone:10.1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), and 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4). Cells were then stained with APC Anti-Human CD11b (ICRF44) (APC-65116, Clone: ICRF44). Cells were not fixed. Monocytes were gated.

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1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Anti-Human B7-H3/CD276 Rabbit Recombinant Antibody (98017-1-RR, Clone: 230305A10) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed.

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1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-MR, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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1x10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human LAMP-2 (H4B4) Mouse Recombinant Antibody (65566-1-MR, Clone:H4B4) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8)(red), or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-RR, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-RR, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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