A375 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Untreated and PNGase F-treated lysates of HepG2 cells and L02 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

WB result of CD146/MCAM antibody (17564-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD146/MCAM transfected HepG2 cells.

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 40x lens).

IP Result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug.

Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HUVEC cells were stained with 0.2ug CD146/MCAM antibody (17564-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human CD146 (65181-1-Ig, Clone:P1H12) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul PE Anti-Human CD146 (PE-65181, Clone: P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul PE Anti-Human CD146 (PE-65181, Clone: P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul Atlantic Blue™ Anti-Human CD146 (AB-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul Atlantic Blue™ Anti-Human CD146 (AB-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul Cardinal Red™ Anti-Human CD146 (CR-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul Cardinal Red™ Anti-Human CD146 (CR-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD146 (CL405-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD146 (CL405-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD146 (CL488-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD146 (CL488-65181, Clone:P1H12), or Mouse IgG1 Isotype Control. Cells were not fixed.