Raji cells (CD20+), Daudi cells (CD20+), Ramos cells (CD20+), Jurkat cells (CD20-), and HeLa cells (CD20-) were subjected to SDS PAGE followed by western blot with 60271-1-Ig (CD20 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green).

1X10^6 Raji cells were stained with 0.20 ug Anti-Human CD20 (60271-1-Ig, Clone:4A7G3) (red) or 0.20 ug Mouse IgG2b Isotype Control (66360-3-Ig, CloneK11B8C4B5) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Cells were fixed with90% MeOH.

Various lysates were subjected to SDS PAGE followed by western blot with 24828-1-AP (CD20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24828-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (24828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red).

IP Result of anti-CD20 (IP:24828-1-AP, 4ug; Detection:24828-1-AP 1:600) with Raji cells lysate 1440ug.

1X10^6 Raji cells were stained with 0.2ug MS4A1,CD20 antibody (24828-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.

1X10^6 human peripheral blood lymphocytes were surface stained with 0.125 ug APC-anti-human CD20 (APC-65085, clone 2H7) (red) or 0.125 ug APC-mouse IgG2b isotype control (blue). Cells were not fixed.

1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD19 and 5 ul Atlantic Blue™ Anti-Human CD20 (AB-65085, Clone:2H7) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD20 (AB-65085, Clone:2H7) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with APC Anti-Human CD19 and 5 ul Cardinal Red™ Anti-Human CD20 (CR-65085, Clone:2H7) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD20 (CR-65085, Clone:2H7) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD19 and 5 ul CoraLite®405 Anti-Human CD20 (CL405-65085, Clone:2H7), or 5 ul Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite®405 Anti-Human CD20 (CL405-65085, Clone:2H7), or 5 ul Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul/test CoraLite®488-conjugated Anti-Human CD20 (CL488-65085, Clone: 2H7) (red) or isotype control antibody (blue). Cells were not fixed.

1X10^6 human PBMCs were surface co-stained with APC Anti-Human CD19 and 5 ul CoraLite® Plus 555 Anti-Human CD20 (CL555-65085, Clone:2H7) or Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD20 (CL555-65085, Clone:2H7) or Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5). Cells were not fixed. Lymphocytes were gated.