1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD24 (65121-1-Ig, Clone: M1/69) and FITC anti-rat IgG2b Antibody at dilution 1:200 (green), or stained with 0.5 ug isotype control and FITC anti-rat IgG2b Antibody at dilution 1:200 (black). Cells were not fixed.

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1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.06 ug APC-Rat IgG2b isotype control (left) or 0.06 ug APC Anti-Mouse CD24 (APC-65121, Clone: M1/69) (right). Cells were not fixed.

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Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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1X10^6 Ramos cells were stained with 0.2 ug Anti-Human CD24 (67627-1-Ig, Clone: 1H5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

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1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-conjugated rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD24 (CL488-65121, Clone: M1/69) (right). Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 5.00 ul CoraLite®647-conjugated Anti-Mouse CD24 (CL647-65121, Clone: M1/69). Cells were not fixed.

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1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and CoraLite®647-conjugated rat IgG2b isotype control antibody. Cells were not fixed.

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1X10^6 BALB/c mouse splenocytes were surface stained with 0.06 ug PE Anti-Mouse CD24 (PE-65121, Clone: M1/69) (red) or 0.06 ug isotype control antibody (black). Cells were not fixed.

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