Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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WB result of CD47 antibody (66304-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD47 transfected A549 cells.

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1X10^6 HUVEC cells were stained with 0.20 ug Anti-Human CD47 (66304-1-Ig, Clone:1E1D8) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

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1X10^6 HUVEC cells were surface stained with 0.2 ug Anti-Human CD47 (29555-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were not fixed .

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1X10^6 HUVEC cells were surface stained with 0.25 ug CoraLite®488 Anti-Human CD47 (CL488-29555) (red), or 0.25 ug Control Antibody. Cells were not fixed .

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1X10^6 HUVEC cells were surface stained with 0.25 ug CoraLite®647 Anti-Human CD47 (CL647-29555) (red), or 0.25 ug Control Antibody. Cells were not fixed .

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1X10^6 HUVEC cells were surface stained with 0.5 ug CoraLite®555 Anti-Human CD47 (CL555-29555) (red), or 0.5 ug Control Antibody. Cells were not fixed .

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