1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-RR, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 Rabbit Recombinant Antibody (65513-1-PBS, Clone:UCHT2) or Rabbit IgG control PolyAb, and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 (65200-1-Ig, Clone:L17F12) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 (65200-1-Ig, Clone:L17F12) with CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-MR, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-MR, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

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1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD5 Mouse Recombinant Antibody (65530-1-PBS, Clone:L17F12) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 (65152-1-Ig, Clone:UCHT2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD5 (65152-1-Ig, Clone:UCHT2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface co-stained with Atlantic Blue™ Anti-Human CD19 and 5 ul APC Anti-Human CD5 (APC-65152, Clone:UCHT2), or PE Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD5 (APC-65152, Clone:UCHT2), or PE Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD19 and 5 ul Atlantic Blue™ Anti-Human CD5 (AB-65200, Clone: L17F12) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD5 (AB-65200, Clone:L17F12) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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