Untreated and PNGase F-treated lysates of Raji cells and HeLa cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

Various lysates were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

1X10^6 Raji cells were stained with 0.2ug CD71 antibody (66180-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-mouse IgG with dilution 1:100.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

IP Result of anti-CD71 (IP:66180-1-Ig, 5ug; Detection:66180-1-Ig 1:1000) with HeLa cells lysate 2400ug.

1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human CD71 (65235-1-Ig, Clone:OKT9) and CL594-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. Blue line indicates unstained stimulated cells. Black dashed line indicates unstimulated stained cells.

1X10^6 PHA-activated human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD71 (AB-65235, Clone:OKT9) (red) or Mouse IgG1 Isotype Control (blue). Unactivated human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD71 (black). Cells were not fixed. Lymphocytes were gated.

1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD71 (CL405-65235, Clone:OKT9) (red) or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD71. Cells were not fixed. Lymphocytes were gated.

1X10^6 PHA-activated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD71 (CL647-65235, Clone: OKT9), or APC Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 PHA-activated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD71 (CL750-65235, Clone:OKT9) (red) or Mouse IgG1 Isotype Control (blue). Unactivated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD71 (black). Cells were not fixed. Lymphocytes were gated.

1X10^6 PHA-activated human PBMC were surface stained with 5 ul FITC Plus Anti-Human CD71 (FITC-65235, Clone: OKT9). Unactivated human PBMC were surface stained with 5ul FITC Plus Anti-human CD71 as the negative control. Cells were not fixed.