1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.
1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.