Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-60330 (Vimentin antibody) at dilution of 1:100.

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1X10^6 HepG2 cells were stained with 0.20ug Vimentin antibody (CL594-60330, red) and control antibody (blue). Fixed with 90% MeOH.

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Various lysates were subjected to SDS PAGE followed by western blot with CL594-60012 (GRP94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated GRP94 antibody (CL594-60012, Clone: 4G7C7 ) at dilution of 1:100.

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1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GRP94 (CL594-60012, Clone:4G7C7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Immunofluorescent analysis of HepG2 cells using 19842-1-AP (hIST1 antibody) at dilution of 1:25 and SA00013-4 CoraLite594-conjugated Goat Anti-Rabbit IgG(H+L).

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Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (66095-1-Ig, Clone: 3C10G12 ) at dilution of 1:400 and SA00013-3 CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Various lysates were subjected to SDS PAGE followed by western blot with CL594-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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1X10^6 HepG2 cells were stained with 0.20ug alpha Tubulin antibody (CL594-66031, red) and control antibody (blue). Fixed with 90% MeOH.

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Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66031 (alpha Tubulin antibody) at dilution of 1:100.

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Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated version of this antibody, CL594-66031 (alpha Tubulin antibody), at dilution of 1:100.

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1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®647-conjugated Anti-Human CD3 (CL647-65151, Clone: UCHT1) and 5 ul CoraLite®594 Anti-Human CD19 (CL594-65110, Clone:HIB19). Cells were not fixed.

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1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1) and CoraLite®594-conjugated Mouse IgG1 isotype control. Cells were not fixed.

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