Various lysates were subjected to SDS PAGE followed by western blot with CL594-60012 (GRP94 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated GRP94 antibody (CL594-60012, Clone: 4G7C7 ) at dilution of 1:100.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GRP94 (CL594-60012, Clone:4G7C7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®647-conjugated Anti-Human CD3 (CL647-65151, Clone: UCHT1) and 5 ul CoraLite®594 Anti-Human CD19 (CL594-65110, Clone:HIB19). Cells were not fixed.
1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1) and CoraLite®594-conjugated Mouse IgG1 isotype control. Cells were not fixed.