Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 4 ul FITC Anti-Human CD206 (FITC-65155, Clone: 15-2) (green) or FITC-Mouse IgG1 isotype control (black). Cells were not fixed.
1X10^6 BALB/c mouse peritoneal macrophages were surface stained with 0.20 ug FITC-Anti-Mouse CD80 (B7-1) (FITC-65076, clone 16-10A1) (red) or 0.20 ug FITC-Armenian Hamster IgG isotype control (blue). Cells were not fixed.
1X10^6 LPS-stimulated (3 days) C57BL/6 mouse splenocytes were surface stained with 0.20ug FITC-Anti-Mouse CD86 (FITC-65068, clone GL1) (red) or 0.20 ug FITC-rat IgG2a isotype control (blue). Cells were not fixed.
1X10^6 HeLa cells were intracellularly stained with 10 μl FITC-Anti-Human CD107a antibody (FITC-65051, clone H4A3) (red) or 10 μl FITC-mouse IgG1 isotype control (blue). Cells were fixed with 90% MeOH.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FITC-65051 (CD107a antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).