1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human MME,CD10 (18008-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP Result of anti-MME,CD10 (IP:18008-1-AP, 3ug; Detection:18008-1-AP 1:500) with Raji cells lysate 2400ug.
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (MME,CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (MME,CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).