Various lysates were subjected to SDS PAGE followed by western blot with 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

WB result of HER2/ErbB2 antibody (18299-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HER2/ErbB2 transfected MCF-7 cells.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 18299-1-AP (HER2/ErbB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 MCF-7 cells were stained with 0.2ug HER2/ErbB2 antibody (18299-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

IP Result of anti-HER2/ErbB2 (IP:18299-1-AP, 5ug; Detection:18299-1-AP 1:500) with SGC-7901 cells lysate 2400ug.