Various lysates were subjected to SDS PAGE followed by western blot with 20103-1-AP (CD45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20103-1-AP (CD45 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD45 antibody (20103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD45 antibody (20103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP result of anti-CD45 (IP:20103-1-AP, 4ug; Detection:20103-1-AP 1:300) with mouse spleen tissue lysate 4800ug.

1X10^6 Jurkat cells were stained with 0.5ug CD45 antibody (20103-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

pig spleen tissue were subjected to SDS PAGE followed by western blot with 60287-1-Ig (CD45 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60287-1-Ig (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD45 antibody (60287-1-Ig, Clone: 4E9B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD45 antibody (60287-1-Ig, Clone: 4E9B2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 human PBMCs were surface stained with 5 ul FITC Anti-Human CD45 (FITC-65109, Clone:HI30) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 10 ul APC Anti-Human CD45 (APC-65064, Clone: F10-89-4) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 10 ul APC Anti-Human CD45 (APC-65064, Clone: F10-89-4) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD45 (PE-65109, Clone:HI30) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45 (65109-1-Ig, Clone:HI30) or Mouse IgG1 Isotype Control and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (65109-1-Ig, Clone: HI30 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 and 0.5 ug Anti-Mouse CD45R (B220) (65139-1-Ig, Clone: RA3-6B2) or 0.5 ug Isotype Control. Cells were not fixed.

Immunofluorescent analysis of mouse splenocytes using CD45R (B220) antibody (65139-1-Ig, Clone: RA3-6B2 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 80297-1-RR (CD45 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).

1X10^6 Raji cells were intracellularly stained with 0.15 ug Anti-Human CD45 (80297-1-RR, Clone: 6O19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.15 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).