1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a / LAMP1 (65051-1-Ig, Clone:H4A3) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107a (CL647-65051, Clone:H4A3) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107a antibody (CL647-65051, Clone: H4A3) at dilution of 1:400. F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).

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1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107b / LAMP2 (65053-1-Ig, Clone:H4B4) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65053-1-Ig (CD107b antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite®488 Anti-Human CD107a (CL488-65051, Clone:H4A3) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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1X10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b (CL647-65053, Clone:H4B4) (red) or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107b antibody (CL647-65053, Clone: H4B4) at dilution of 1:50 and CoraLite®488-conjugated CD107a antibody (CL488-65051, Clone: H4A3, green) at dilution of 1:50.

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Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated CD107a antibody (CL594-65051, Clone: H4A3) at dilution of 1:400. F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).

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1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD107a / LAMP1 (CL594-65051, Clone:H4A3)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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1X10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite®594 Anti-Human CD107b (CL594-65053, Clone:H4B4) (red) or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated CD107b antibody (CL594-65053, Clone: H4B4) at dilution of 1:50.

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Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FITC-65051 (CD107a antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD107a (FITC-65051, Clone: H4A3). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

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1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD107a (FITC-65051, Clone: H4A3). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

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