Results for "Neural stem cells"

109 product results for:

"Neural stem cells"

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Neural stem cells

Neural stem cells (NSCs) have the ability to differentiate into various neural cell types, including neurons, astrocytes, and oligodendrocytes. NSC-based therapies hold promise for treating conditions like Parkinson's disease, motor neuron diseases, and spinal cord injury by replacing damaged or lost neural cells and promoting neural repair.

Cytokines & Growth Factors

Antibodies for Characterization

Other Reagents

Nestin Polyclonal antibody

76 Publications

Reviews (4)

View More Images (6)

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Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nestin antibody (19483-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IF, FC, IHC, ELISA

Sizes
-_- / -_-

Cat No. 19483-1-AP
×

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More validation images available on the product page

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nestin antibody (19483-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of U-251 cells using 19483-1-AP (Nestin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 19483-1-AP (Nestin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with sox2 (66411-1-Ig, red).

Mouse brain lysates were subjected to SDS PAGE followed by western blot with 19483-1-AP (Nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 10x lens).
PAX6 Polyclonal antibody

KO/KD Validated

56 Publications

Reviews (3)

View More Images (7)

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Immunofluorescent staining of PAX6 (12323-1-AP, 1:250 dilution) with 4% PFA fixed control human induced pluripotent stem cells (hiPSC) derived neuronal precursor cells (NPCs). (Green: PAX6; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Sizes
-_- / -_-

Cat No. 12323-1-AP
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More validation images available on the product page

Immunofluorescent staining of PAX6 (12323-1-AP, 1:250 dilution) with 4% PFA fixed control human induced pluripotent stem cells (hiPSC) derived neuronal precursor cells (NPCs). (Green: PAX6; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for PAX6 with 12323-1-AP at 1:600 (green), and SOX2 with 11064-1-AP at 1:200 (red), Nuclear stain DAPI (blue). Scale bar = 20 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and PAX6 (12323-1-AP) at 1:500. Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

Various lysates were subjected to SDS PAGE followed by western blot with 12323-1-AP (PAX6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PAX6 (12323-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
TUBB3-specific/TUJ1 Monoclonal antibody

49 Publications

Reviews (3)

View More Images (13)

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Immunofluorescent staining of TUBB3 (66375-1-lg, 1:250) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (Green: TUBB3; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat, chicken and More (2)

Applications

WB, IF, FC, IHC, ELISA

CloneNo.

1F8G10

Sizes
-_- / -_-

Cat No. 66375-1-Ig
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Immunofluorescent staining of TUBB3 (66375-1-lg, 1:250) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (Green: TUBB3; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and PAX6 (12323-1-AP) at 1:500. Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue).

Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66375-1-Ig (TUBB3-specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 26975-1-AP (NeuN antibody, red).

Various lysates were subjected to SDS PAGE followed by western blot with 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:49000 incubated at room temperature for 1.5 hours.

1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human TUBB3-specific (66375-1-Ig, Clone:1F8G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 40x lens).

Western blot analysis of TUBB3 in various tissues and cell lines with 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Nestin Monoclonal antibody

32 Publications

View More Images (2)

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Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Host/IsoType

Mouse/IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

CloneNo.

1D11H2

Sizes
-_- / -_-

Cat No. 66259-1-Ig
×

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Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
p63 Polyclonal antibody

KO/KD Validated

29 Publications

Reviews (2)

View More Images (6)

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Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, CoIP, ChIP, ELISA

Sizes
-_- / -_-

Cat No. 12143-1-AP
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed A431 cells using p63 antibody (12143-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

IP result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:1000) with A431 cells lysate 1200 ug.

1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human p63 (12143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
OTX2 Polyclonal antibody

KO/KD Validated

27 Publications

Reviews (1)

View More Images (6)

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Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 13497-1-AP (OTX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse, rat, chicken

Applications

WB, IP, IF, FC, IHC, CoIP, ChIP, ELISA

Sizes
-_- / -_-

Cat No. 13497-1-AP
×

The search results gallery shows a selection of our total image library
More validation images available on the product page

Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 13497-1-AP (OTX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 13497-1-AP (OTX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 13497-1-AP (OTX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 40x lens).

IP result of anti-OTX2 (IP:13497-1-AP, 3ug; Detection:13497-1-AP 1:1500) with Y79 cells lysate 2400ug.

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human OTX2 (13497-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
CoraLite®594-conjugated TUBB3-specific/TUJ1 Monoclonal antibody

2 Publications

Reviews (1)

View More Images (5)

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Neuro-2a lysates were subjected to SDS PAGE followed by western blot with CL594-66375 (TUBB3-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Host/IsoType

Mouse/IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra)

Conjugate

CoraLite®594

CloneNo.

1F8G10

Sizes
-_- / -_-

Cat No. CL594-66375
×

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More validation images available on the product page

Neuro-2a lysates were subjected to SDS PAGE followed by western blot with CL594-66375 (TUBB3-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66375 (TUBB3-specific antibody) at dilution of 1:50.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL594-66375 (TUBB3-specific antibody) at dilution of 1:50.

1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TUBB3-specific (CL594-66375, Clone:1F8G10) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TUBB3-specific antibody (CL594-66375, Clone: 1F8G10 ) at dilution of 1:200.
GBX2 Polyclonal antibody

KO/KD Validated

2 Publications

View More Images (4)

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WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells.

Host/IsoType

Rabbit/IgG

Reactivity

human, mouse

Applications

WB, IP, IF, chIP, ELISA

Sizes
-_- / -_-

Cat No. 21639-1-AP
×

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More validation images available on the product page

WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells.

mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21639-1-AP (GBX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IP Result of anti-GBX2 (IP:21639-1-AP, 4ug; Detection:21639-1-AP 1:300) with HeLa cells lysate 3000ug.

Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21639-1-AP (GBX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).