human placenta tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Untreated and IFN gamma treated A375 cells and A549 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66248-1-Ig(PD-L1/CD274 antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 MDA-MB-231 cells were stained with 0.2 ug Anti-Human PD-L1/CD274 (66248-1-Ig, Clone: 2B11D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug mosue IgG1 isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul APC Anti-Human PD-L1 (B7-H1) (APC-65081, Clone: 29E.2A3) (blue) or isotype control antibody (black). Cells were not fixed.

1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human PD-L1 (B7-H1) (65081-1-Ig, Clone: 29E.2A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug mouse IgG2b isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20ug APC-Anti-Mouse CD274 (PD-L1, B7-H1) (APC-65073, clone 10F.9G2) (red) or 0.20 ug APC-Rat IgG2b isotype control (blue). Cells were not fixed.

1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul CoraLite® Plus 488 Anti-Human PD-L1 (B7-H1) (CL488-65081, Clone: 29E.2A3) (green) or isotype control (black). Cells were not fixed.

1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul CoraLite®647 Anti-Human PD-L1 (B7-H1) (CL647-65081, Clone: 29E.2A3) (blue) or isotype control (black). Cells were not fixed.

1X10^6 mouse splenocytes cells were stained with 0.20ug CD274 (PD-L1, B7-H1) antibody (PE-65073, red) and control antibody (blue). Cells were not fixed.

human placenta tissue were subjected to SDS PAGE followed by western blot with 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Untreated and IFN gamma treated A549 cells were subjected to SDS PAGE followed by western blot with 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of HEK-293 cells using 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP Result of anti-PD-L1/CD274 (IP:17952-1-AP, 4ug; Detection:66248-1-Ig 1:1000) with mouse heart tissue lysate 4000ug.

1X10^6 Raji cells were stained with 0.20ug PD-L1/CD274 antibody (17952-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.