Left: HeLa cells transiently expressing pSpot Spot-Tag?-Actin (ev-31) were immunostained with Spot-Label? Alexa Fluor? 647 (magenta, ebAF647, 1:800) and DAPI (blue). Right: Control staining, no background noise; HeLa cells without pSpot Spot-Tag?-Actin expression were immunostained with Spot-Label? Alexa Fluor? 647 (magenta, ebAF647, 1:800) and DAPI (blue). Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective.
