Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®594 ZO-1 antibody (CL594-21773) at dilution of 1:200, CL488-phalloidin (green).

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1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ZO-1 (CL594-21773) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite®488 ZO-1 antibody (CL488-21773) at dilution of 1:200.

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Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®488 ZO-1 antibody (CL488-21773) at dilution of 1:200.

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1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human ZO-1 (CL488-21773) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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