Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .
E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.
Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.