WB result of E-cadherin antibody (20874-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells.
E14.5 FFPE mouse embryo section stained for E-cadherin (red, Cat. No 20874-1-AP) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see the epithelial layer of tissue clearly stained, particularly the skin, eyes, and respiratory system. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
T-47D cells were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HepG2 cells were stained with .2ug E-cadherin antibody (20874-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP Result of anti-E-cadherin (IP:20874-1-AP, 3ug; Detection:20874-1-AP 1:1000) with A431 cells lysate 3000ug.
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of A431 cells using 20874-1-AP (E-cadherin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).