Various lysates were subjected to SDS PAGE followed by western blot with CL488-66240 (Beta Tubulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66240 (beta Tubulin antibody) at dilution of 1:100.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Beta Tubulin (CL488-66240, Clone:1D4A4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 TOM20 antibody (CL488-11802) at dilution of 1:200, CL594-Phalloidin (red).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOM20 (CL488-11802) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 DYKDDDDK tag antibody (CL647-80010, Clone: 4K14 ) at dilution of 1:200.
Various lysates were subjected to SDS PAGE followed by western blot with CL488-60004 (GAPDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with CL488-60004 (GAPDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GAPDH (CL488-60004, Clone:1E6D9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Various lysates were subjected to SDS PAGE followed by western blot with CL488-66009 (Beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 Beta Actin antibody (CL488-66009, Clone: 2D4H5 ) at dilution of 1:200.
(blue)1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Beta Actin (CL488-66009, Clone:2D4H5)(red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD206 antibody (CL488-60143, Clone: 2A6A10 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD206 antibody (CL488-60143, Clone: 2A6A10 ) at dilution of 1:200.
1X10^6 mouse splenocytes were surface co-stained with CoraLite®594 Anti-Mouse CD3 and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD8a (CL488-65069, Clone: 53-6.7) or 0.5 ug Isotype Control. Cells were not fixed.
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD8a (CL488-65069, Clone: 53-6.7) at dilution of 1:500.
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 GFAP antibody (CL488-60190, Clone: 4B2E10 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 GFAP antibody (CL488-60190, Clone: 4B2E10 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11 ) at dilution of 1:100, CoraLite®488 Cardiac Troponin I antibody (CL488-66376, Clone: 1D5D6, green), CoraLite®594 N-cadherin antibody (CL594-22018, red). DAPI (blue).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11 ) at dilution of 1:200.
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human smooth muscle actin specific (CL647-67735, Clone:1E9A11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66005 (6*His, His-Tag antibody), at dilution of 1:100.
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL488-66200 (GREEN, Acetyl-Tubulin (Lys40) antibody) at dilution of 1:50 and CL594-17711 (RED, ARL13B antibody) at dilution of 1:50.
Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD-31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD-31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB.
E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66031 (alpha Tubulin antibody), at dilution of 1:100.
Various lysates were subjected to SDS PAGE followed by western blot with CL488-66031 (Alpha Tubulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Alpha Tubulin (CL488-66031, Clone:1E4C11) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66467 (Heavy chain of Rabbit IgG antibody), at dilution of 1:400.
1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD163 (CL488-65169, Clone:GHI/61) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD163 (CL488-65169, Clone:GHI/61) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-65124). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.
1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul CoraLite® Plus 488-conjugated Anti-Human CD29 (CL488-65191, Clone: TS2/16) (red) or isotype control antibody (blue). Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11b (CL488-65055, Clone:M1/70), and 0.25 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2), and 0.25 ug APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (APC-65140, Clone: RB6-8C5). Cells were not fixed .
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD11b (CL488-65055, Clone: M1/70) at dilution of 1:400.
1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD4 (RM4-5) (CL488-65141, Clone:RM4-5) or 0.25 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5) at dilution of 1:500.
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ACE2 antibody (CL488-66699, Clone: 2F12A4 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ACE2 antibody (CL488-66699, Clone: 2F12A4 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD11c/Integrin Alpha X antibody (CL488-60258, Clone: 2F1C10 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD11c/Integrin Alpha X antibody (CL488-60258, Clone: 2F1C10 ) at dilution of 1:200.
Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-66065, Clone: 2A1E2 ) at dilution of 1:400.