1X10^6 C57BL/6 mouse splenocytes were surface stained with 1.00 ug FITC Anti-Mouse CD31 (FITC-65058, Clone: 390) (red) or 1.00 ug isotype control antibody (blue). Samples were not fixed.
1X10^6 Raji cells were surface stained with 0.50 ug Anti-Human CD40 (65103-1-Ig, Clone: G28.5) (red) or 0.50 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Samples were not fixed.
1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone: AD2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution of 1:1000 (red), or with 0.5 ug mouse IgG1 isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution of 1:1000 (blue). Samples were not fixed.
1X10^6 Daudi cells were surface stained with 0.20 ug Anti-Human CD86 (65165-1-Ig, Clone: BU63) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Samples were not fixed.
1X10^6 HeLa cells were surface stained with 0.20 ug/test Purified Anti-Human MICA/MICB (65161-1-Ig, Clone:6D4) (red) or 0.20 ug Mouse IgG2a Isotype Control (66360-2-Ig, CloneK11A1B2A2) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Samples were not fixed.
1X10^6 human peripheral blood granulocytes were surface stained with 5.00 ul APC Anti-Human CD11b (APC-65116, Clone:ICRF44) (red) or isotype control antibody (blue). Samples were not fixed.
100 ul human peripheral blood were surface stained with 5.00 ul APC Anti-Human CD11c (APC-65086, Clone:3.9) and then treated with red blood cell lysis buffer. Cells were not fixed.
100 ul human peripheral blood were surface stained with 10.00 ul APC Anti-Human CD163 (APC-65169, Clone: GHI/61) or APC mouse IgG1 isotype control antibody. CD14+ monocytes were gated for analysis. Samples were not fixed.
