1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite®647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 1 ug Anti-Mouse CD197 (65158-1-Ig, Clone: 4B12) and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite®647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 1 ug Rat IgG2a Isotype Control and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.