1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD4 (APC-65143, Clone: RPA-T4), 1 ug Anti-Human CD25 (65096-1-Ig, Clone: BC96) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.
1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD4 (APC-65143, Clone: RPA-T4), 1 ug Mouse IgG1 Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.