1X10^6 C57BL/6 mouse splenocytes were surface stained with 1.00 ug FITC Anti-Mouse CD31 (FITC-65058, Clone: 390) (red) or 1.00 ug isotype control antibody (blue). Samples were not fixed.
1X10^6 Human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD45RO (65150-1-Ig, Clone:UCHL1) (red) or 0.25 ug Mouse IgG2a Isotype Control(66360-2-Ig, Clone:K11A1B2A2) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Samples were not fixed.
1X10^6 Daudi cells were surface stained with 0.20 ug Anti-Human CD86 (65165-1-Ig, Clone: BU63) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Samples were not fixed.
1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse Ly-6G (Gr-1) (65140-1-Ig, Clone: RB6-8C5) (red) or 0.25 ug isotype control antibody (blue) and Fluorescein (FITC)–conjugated Affinipure Goat Anti-Rat IgG(H+L) with dilution 1:1000. Samples were not fixed.
1X10^6 human peripheral blood granulocytes were surface stained with 5.00 ul APC Anti-Human CD11b (APC-65116, Clone:ICRF44) (red) or isotype control antibody (blue). Samples were not fixed.
1X10^6 human peripheral blood granulocytes were surface stained with 10.00 ul/test APC Anti-Human CD13 (APC-65186, Clone:WM15) (red) or isotype control antibody (blue). Samples were not fixed.
100 ul human peripheral blood were surface stained with 10.00 ul APC Anti-Human CD163 (APC-65169, Clone: GHI/61) or APC mouse IgG1 isotype control antibody. CD14+ monocytes were gated for analysis. Samples were not fixed.
1X10^6 human peripheral blood lymphocytes were surface stained with 0.125 ug FITC-Anti-Human CD4 (FITC-65143, clone RPA-T4) and 0.125 ug APC-mouse IgG1 isotype control. Samples were not fixed.
1X10^6 human peripheral blood lymphocytes were surface stained with 0.125 ug FITC-Anti-Human CD4 (FITC-65143, clone RPA-T4) and 0.125 ug APC-Anti-Human CD25 (APC-65096, clone BC96). Samples were not fixed.
