Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

Immunoprecipitation of GFP with GFP-Trap Agarose beads. I: Input, FT: Flow-Through, B: Bound.

GFP-Trap Magnetic Agarose for immunoprecipitation of GFP-fusion proteins. Note single-band in bound fraction, which indicates a very effective pull-down. I: Input, FT: Flow-Through, B: Bound.

Immunoprecipitation of mCherry fusion with RFP-Trap.I: Input, FT: Flow-through, B: Bound.

Myc-Trap Agarose for immunoprecipitation of Myc-tagged proteins.

Immunoprecipitation of GFP with GFP-Trap Agarose beads. I: Input, FT: Flow-Through, B: Bound.

The ubiquitin-trap magnetic agarose (utma) was used to immunoprecipitate endogenous ubiquitin and ubiquitinylated proteins from human (HEK293T), mouse (C2C12), and hamster (CHO) cell lines treated with MG-132. For each IP, samples of the input lysate (I), non-bound flow-through (F), and bound (B) fractions were analyzed using western blot. Ubiquitin recombinant antibody (80992-1-RR) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (SA00001-2) were used in the western blot analysis.

The ubiquitin-trap magnetic agarose (utma) was used to immunoprecipitate endogenous ubiquitin and ubiquitinylated proteins from dog (MDCK) cells, spinach (Spinacia oleracea), and baker's yeast (Saccharomyces cerevisiae) treated with MG-132. For each IP, samples of the input lysate (I), non-bound flow-through (F), and bound (B) fractions were analyzed using western blot. Ubiquitin recombinant antibody (80992-1-RR) and HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) (SA00001-2) were used in the western blot analysis.