Various lysates were subjected to SDS PAGE followed by western blot with Biotin-60293 (OPTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SA00001-0 (HRP-conjugated Streptavidin) as secondary antibody, at dilution of 1:3000 (lane 1 and 3) or 1:6000 (lane 2 and 4).
