IHC and IF Analysis Workshop

Workshop led by Dr Lukasz Chrobok, a Research Fellow from the University of Bristol

A key aspect of many IF/IHC experiments is performing the correct analyses of your images to collect meaningful data. It can be daunting to know where to start and which way is best for you.

What this video will cover:

  • Image analysis with ImageJ/FIJI for beginners
  • Types of images (color channels, stacks)
  • Image processing
  • Measuring intensity
  • Measuring area fraction (binarization)
  • Thresholding
  • Adaptive thresholding (e.g. Bernsen filter)