|Positive WB detected in||Recombinant protein protein|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
HRP-66005 targets 6*His, His-Tag in WB applications and shows reactivity with recombinant protein samples.
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Apoorv (Verified Customer) (12-15-2019)
Used to detect protein in Western blots
Soo (Verified Customer) (10-24-2019)
we use this antibody to detect recombinant proteins which has his tag. Used it for western and ELISA and Flow , works well for us. It is cheaper than other brand's his tag antibody, another reason we like this product.
Angel (Verified Customer) (09-09-2019)
It is the best antibody I've ever used for quick check of protein expression and size for heterologous protein production and purification.
Ryan (Verified Customer) (02-04-2019)
This His-tag-HRP conjugated antibody is the single best His-tag antibody I have ever used. I have used dilutions from 1:1000 to 1:25,000 with fantastic results. The background with this antibody is minimal and the sensitivity is superb. This image shows four different proteins with both 6xHis and 8xHis-tags. I have used the antibody in milk and 5% BSA. Incubation times have been as short as 1 hour and as long as 24 hours; the results are always the same. Being conjugated, it can cut the time of a typical Western blot down by a few hours. My standard procedure with this antibody is 1:8,000 in 5% BSA for 1 hour with three 10 minute washes and a 5 second exposure using Cell Signaling SignalFire ECL.
Jennifer (Verified Customer) (12-08-2018)
Excellent product. We achieved to see the production of our protein of interest in an accurate way. Some previous secondary antibodies gave us a lot of background; this one works better.