Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgM


human, mouse, rat, plant, Zebrafish and More (8)







Cat no : 60008-1-Ig


ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin

Tested Applications

Positive WB detected inmulti-cells/tissue, HeLa cells, MCF-7 cells, HEK-293 cells, A549 cells, rice whole plant tissue, arabidopsis whole plant tissue
Positive IHC detected inhuman colon tissue, human brain tissue, human kidney tissue, human lung tissue, human spleen tissue, human ovary tissue, human testis tissue, human placenta tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60008-1-Ig targets Beta Actin in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, plant, Zebrafish samples.

Tested Reactivity human, mouse, rat, plant, Zebrafish
Cited Reactivityhuman, rat, cat, goat, tick, chicken, yeast, Arabidopsis, Terminalia bellirica, Rare minnow
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Beta Actin fusion protein Ag0297
Full Name actin, beta
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC002409
Gene Symbol Beta Actin
Gene ID (NCBI) 60
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.


Product Specific Protocols
WB protocol for Beta Actin antibody 60008-1-IgDownload protocol
IHC protocol for Beta Actin antibody 60008-1-IgDownload protocol
IF protocol for Beta Actin antibody 60008-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu


A phase-separated nuclear GBPL circuit controls immunity in plants.

Authors - Shuai Huang


PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte

Cell Metab

Microenvironmental ammonia enhances T cell exhaustion in colorectal cancer

Authors - Hannah N Bell

Nat Genet

Simultaneous disruption of PRC2 and enhancer function underlies histone H3.3-K27M oncogenic activity in human hindbrain neural stem cells.

Authors - Gerard L Brien


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Udesh (Verified Customer) (08-16-2023)

The Ab worked well for both WB and IF at mentioned dilutions

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
  • Cell Tissue Type: Glioblastoma cells

Chun (Verified Customer) (09-07-2020)

This is an excellent antibody for immunoblotting.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HEK293, HeLa, HaCaT and SH-SY5Y cells

Nikhil (Verified Customer) (09-17-2019)

Very good and specific. I can use 1:2500 5 ml antibody solution two times.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: Colon cancer cells
Beta Actin Antibody Western Blot, validation (1:2500 dilution) in Colon cancer cells (Cat no:60008-1-Ig)

Leonardo (Verified Customer) (09-12-2019)

Excellent antibody. Works well since the first time.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5,000
  • Cell Tissue Type: Human breast cancer (MDA-MB-231)
Beta Actin Antibody Western Blot, validation (1:5,000 dilution) in Human breast cancer (MDA-MB-231) (Cat no:60008-1-Ig)

mark (Verified Customer) (02-05-2019)

Antibody works beautifully to detect a single Beta-actin band as a control

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human cancer cells
Beta Actin Antibody Western Blot, validation (1:2000 dilution) in human cancer cells (Cat no:60008-1-Ig)